Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric enzymes of Pyc1 and Pyc2 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ATP | 30°C, pH 7.8 | Saccharomyces cerevisiae | |
0.5 | - |
pyruvate | 30°C, pH 7.8 | Saccharomyces cerevisiae | |
1.36 | - |
HCO3- | 30°C, pH 7.8 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | Saccharomyces cerevisiae | Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate | ADP + phosphate + oxaloacetate | - |
? | |
ATP + pyruvate + HCO3- | Saccharomyces cerevisiae DM18 | Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate | ADP + phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P11154 | strain DM18 | - |
Saccharomyces cerevisiae | P32327 | - |
- |
Saccharomyces cerevisiae DM18 | P32327 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate | Saccharomyces cerevisiae | ADP + phosphate + oxaloacetate | - |
? | |
ATP + pyruvate + HCO3- | Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate | Saccharomyces cerevisiae DM18 | ADP + phosphate + oxaloacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pyc1 | - |
Saccharomyces cerevisiae |
PYC2 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
ATP | 30°C, pH 7.8 | Saccharomyces cerevisiae | |
60 | - |
pyruvate | 30°C, pH 7.8 | Saccharomyces cerevisiae | |
60 | - |
HCO3- | 30°C, pH 7.8 | Saccharomyces cerevisiae |